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乳业科学与技术 ›› 2019, Vol. 42 ›› Issue (3): 19-23.DOI: 10.15922/j.cnki.jdst.2019.03.004

• 分析检测 • 上一篇    下一篇

高分辨四极杆飞行时间质谱仪判别复原乳和超高温灭菌乳

崔婧, 苏美丞, 谭冬飞, 张霞, 贾曼   

  1. 中国农业科学院农业质量标准与检测技术研究所, 农业部农产品质量安全重点实验室, 北京 100081
  • 出版日期:2019-05-01 发布日期:2021-11-09
  • 基金资助:
    国家国际科技合作专项（2015DFG31890）

A Quadrupole-Time of Flight Mass Spectrometry Method for the Discrimination of Ultra-High Temperature Milk from Reconstituted Milk

CUI Jing, SU Meicheng, TAN Dongfei, ZHANG Xia, JIA Man   

  1. (Key Laboratory of Agrifood Safety and Quality, Ministry of Agriculture, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China)
  • Online:2019-05-01 Published:2021-11-09

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摘要： 采用高分辨四极杆飞行时间质谱仪，结合化学计量学方法，利用非靶向代谢组学方法，对超高温灭菌乳和 复原乳进行检测。牛乳样品经过前处理后，经过C18色谱柱分离，采用Full Scan模式进行一级全扫描，扫描结果通过 数据预处理后，导入SIMCA-P 14.1软件中进行主成分分析和偏最小二乘方差判别分析。结果表明：正、负离子模式 下共找到14 种判别超高温灭菌乳和复原乳的表征因子，进一步通过这14 种表征因子建立区分2 种乳的判别模型， 通过该判别模型能够准确地区分超高温灭菌乳和复原乳，为复原乳的判别提供理论依据。

关键词: 高分辨四极杆飞行时间质谱仪；超高温灭菌乳；复原乳；化学计量学；判别模型

Abstract: In this study, ultra-high temperature (UHT) milk and reconstituted milk were discriminated by high resolution quadrupole-time of flight mass spectrometry (LC-Q-TOF-MS) combined with chemometrics based on non-targeted metabolomic approaches. Sample pretreatment was performed prior to chromatographic separation on a C18 column, and the mass spectrometer was operated in the full scan mode. The obtained data were preprocessed before being imported into SIMCA-P 14.1 for principal component analysis (PCA) and partial least squares-discrimination analysis (PLS-DA). A total of 14 characterization factors were selected in both positive and negative ion modes to establish a discriminant model. The results showed that this model could be used as a robust approach to discriminate between UHT and reconstituted milk and provide a theoretical basis for the identification of reconstituted milk.

Key words: high-resolution quadrupole-time of flight mass spectrometry; ultra-high temperature milk; reconstituted milk; chemometrics; discriminant model

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