The formulation of donkey hide glue containing aqueous from edible flowers and skim goat milk was optimized using combination of one-factor-at-a-time method and response surface methodology with Box-Behnken design.The response variables were sensory evaluation scores and gel strength.The results showed that the optimum formulation was composed of 62.4％ skim goat milk,6.2％ donkey hide glue,aqueous flower extract 14.9％,and 0.075％ composite gelling agent.The resultant product showed a sensory score of 9.6 and a gel strength of 35.8 g.Physicochemical analysis indicated that the product contained ≥ 6.80 g of protein and ≤4.00 g of fat per 100 g of sample and thus could nourish blood and maintain beauty as a high-protein,low-fat product.

As an enzyme that catalyzes the transfer of acyl groups, transglutaminase is widely used in the dairy industry. This paper briefly describes the source and catalytic mechanism of the enzyme, and further explains the mechanism of action of the enzyme on milk proteins, including casein, whey protein and milk protein products. Moreover, the application of the enzyme in the dairy industry is summarized in this paper.