An isotope internal standard coupled with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was established for the simultaneous determination of four bisphenols (bisphenols A, B, F and S) in infant foods. Samples were extracted with a mixture of ethyl acetate and cyclohexane and detected by HPLC-MS/MS in the multi-reaction monitoring mode. Under the optimized conditions, the four bisphenols showed good linearity in the range of 5.0–200.0 μg/L with R2 larger than 0.9970. The detection limit of the method was 1.5 μg/kg. The mean recoveries of negative samples spiked at three concentration levels (5.0, 10.0 and 50.0 μg/L) were in the range of 84.0％–106.5％with relative standard deviations (RSDs, n = 6) ranging from 1.6％ to 6.4％. The proposed method had the advantages of high sensitivity, good purification efficiency and quantitative accuracy and it was suitable for the detection of bisphenols in infant foods.