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Subspecies Identification of Leuconostoc mesenteroides BD1710 Using Specific Primers
GAO Caixia, YAN Minghui, WU Zhengjun
Journal of Dairy Science and Technology
2016, 39 (1):
5-7.
DOI: 10.15922/j.cnki.jdst.2016.01.002
Leuconostoc mesenteroides consists of four subspecies, L. mesenteroides subsp. mesenteroides, L. mesenteroides subsp. dextranicum, L. mesenteroides subsp. suionicum and L. mesenteroides subsp. cremoris. Among these subspecies, only L. mesenteroides subsp. mesenteroide is currently listed for use as a food additive by China’ Ministry of Health and the FDA. The subspecies of L. mesenteroides subsp. mesenteroide cannot be identified simply by 16S rRNA gene sequencing. Therefore, the present study investigated subspecies identification of L. mesenteroides BD1710 using the established primers based on 16S–23S rRNA gene intergenic spacer region sequence similarity and three specific housekeeping genes. The results confirmed the strain to belong to L. mesenteroides subsp. mesenteroides.
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