Streptomyces albulus UN2-71, an ε-Polylysine-Producing Strain: Physiological Characteristics and Optimization of Fermentation Conditions

doi:10.15922/j.cnki.jdst.2013.06.001

Journal of Dairy Science and Technology ›› 2013, Vol. 36 ›› Issue (6): 1-5.DOI: 10.15922/j.cnki.jdst.2013.06.001

• Biotechnology • Next Articles

Streptomyces albulus UN2-71, an ε-Polylysine-Producing Strain: Physiological Characteristics and Optimization of Fermentation Conditions

WANG Gang, HANG Feng, XING Jia-li, TIAN Feng-wei, LIU Xiao-ming, ZHANG Qiu-xiang, ZHANG Hao, CHEN Wei

(1. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; 2. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; 3. State Key Laboratory of Dairy Biotechnology, Bright Dairy & Food Co. Ltd., Shanghai 200436, China)

Online:2013-12-30 Published:2022-02-22

ε-聚赖氨酸生产菌白色链霉菌UN2-71生理特性及其发酵条件优化

王刚, 杭锋, 邢家溧, 田丰伟, 刘小鸣, 张秋香, 张灏, 陈卫

江南大学食品学院,江苏无锡,214122;光明乳业股份有限公司乳业生物技术国家重点实验室,上海,200436;江南大学食品学院,江苏无锡 214122;江南大学食品科学与技术国家重点实验室,江苏无锡 214122

基金资助:
“十二五”国家科技支撑计划项目(2012BAD28B07, 2012BAD28B08)

Abstract

Abstract: ε-Polylysine is widely used as a natural biological preservative in meat, dairy products and other foods for its characteristics of high safety, stability, efficiency, solublity in water and broad-spectrum antibacterial activity. In this paper, physiological and biochemical characteristics of Streptomyces albulus UN2-71, a strain capable of producing ε-polylysine, were studied. The ε-polylysine production increased from 1.64 to 2.37 g/L after optimization of fermentation conditions. The optimized conditions were defined as: 30 mL of the liquid medium in a 250 mL shake flask, inoculum size 10%, temperature 30 ℃, shaking speed 150 r/min and initial fermentation pH 7.0.

Key words: ε-polylysine; Streptomyces albulus; biological preservative; fermentation; optimization 中图分类号：TS 201.3 文献标志码：A 文章编号：1671-

摘要： 以实验室保藏的高产ε-聚赖氨酸白色链霉菌UN2-71为生产菌株,研究其生理生化特性,以此指导新的ε-聚赖氨酸产生菌筛选;同时通过对发酵培养基以及摇瓶发酵条件的优化,最终确定白色链霉菌UN2-71的最佳摇瓶装液量30mL、最佳接种量10％、最佳发酵温度30℃、菌丝生长的最佳摇床转速150r/min、最适起始发酵pH值为7.0.经优化后,白色链霉菌UN2-71的ε-聚赖氨酸产量提高至2.37g/L,较优化之前的产量1.64g/L提高了44.5％.

关键词: ε-聚赖氨酸；白色链霉菌；生物防腐剂；发酵；优化

CLC Number:

TS201.3

WANG Gang, HANG Feng, XING Jia-li, TIAN Feng-wei, LIU Xiao-ming, ZHANG Qiu-xiang, ZHANG Hao, CHEN Wei. Streptomyces albulus UN2-71, an ε-Polylysine-Producing Strain: Physiological Characteristics and Optimization of Fermentation Conditions[J]. Journal of Dairy Science and Technology, 2013, 36(6): 1-5.

王刚, 杭锋, 邢家溧, 田丰伟, 刘小鸣, 张秋香, 张灏, 陈卫. ε-聚赖氨酸生产菌白色链霉菌UN2-71生理特性及其发酵条件优化[J]. 乳业科学与技术, 2013, 36(6): 1-5.

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Streptomyces albulus UN2-71, an ε-Polylysine-Producing Strain: Physiological Characteristics and Optimization of Fermentation Conditions

ε-聚赖氨酸生产菌白色链霉菌UN2-71生理特性及其发酵条件优化

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