Abstract: Objective: A loop-mediated isothermal amplification (LAMP) assay was designed for rapid and accurate detection of Bacillus cereus in yogurt. Methods: Two pairs of oligonucleotide primers exclusively amplifying the hblA gene of Bacillus cereus were designed and Bacillus cereus could be directly detected by LAMP in yogurt, and the amplification products were detected by electrophoresis. Results: Detection of Bacillus cereus in yogurt by the LAMP assay was highly specific, the sensitivity was 6.4 CFU/mL, and the detection limit was 21 CFU/mL. Conclusion: The LAMP was fast, sensitive and useful for the detection of Bacillus cereus in yogurt.

Key words: yogurt；loop-mediated isothermal amplification (LAMP)；detection；Bacillus cereus

摘要： 目的:建立快速准确检测酸乳中蜡样芽孢杆菌的方法.方法:根据蜡样芽孢杆菌hblA基因设计特异性引物,通过环介导等温扩增技术(LAMP)直接检测酸乳中蜡样芽孢杆菌,扩增产物电泳检测.结果:LAMP法检测酸乳中蜡样芽孢杆菌的特异性强,方法检出限6.4CFU/mL,人工污染检出限21CFU/mL.结论:LAMP法用于检测酸乳中蜡样芽孢杆菌的灵敏度高、耗时短,为酸乳中蜡样芽孢杆菌的快速检测提供了新的方法.

关键词: 酸乳；环介导等温扩增；检测；蜡样芽孢杆菌