Abstract: Lactulose as a prebiotic is widely used in milk-based products, especially in infant formula as an important component. For regulation, the Chinese national standard Food Additive-Lactulose Liquid GB8816—1988 has been relatively backward. And there is no regulation regarding the analysis of lactulose in foods. How to discriminate lactulose from other carbohydrates, especially from lactose, is a problem we are faced with and an obstacle for application. The chromatographic techniques for the determination of lactulose in milk-based products, including thin-layer chromatography, gas chromatography and liquid chromatography, are summarized. This work could provide a reference for lactulose detection in milk-based product by researchers.

Key words: lactulose; lactose; chromatographic determination; milk-based product; infant formula

摘要： 异构化乳糖作为益生元在国际乳制品领域中广泛应用,特别是在婴幼儿配方乳粉中作为重要的组成成分.但是我国仅有GB 8816-1988《食品添加剂异构化乳糖液》,且原料的分析方法相对有些滞后.同时也没有相关的食品中异构化乳糖的检测标准.如何将异构化乳糖从其他碳水化合物中区别开来,尤其是与乳糖区分,是目前我国分析检测的一个难点,也是限制异构化乳糖在我国应用的一个障碍.本文主要介绍了国内外关于乳制品中异构化乳糖的色谱检测方法(薄层色谱法、液相色谱法和气相色谱法),以期能为乳制品中异构化乳糖的检测提供参考依据.

关键词: 异构化乳糖；乳糖；色谱检测法；乳制品；婴儿配方乳粉