Abstract: A method for the determination of nucleotides in milk powder by high performance liquid chromatography (HPLC) was developed. Nucleotides were extracted with water, deproteinated with 0.5% acetic acid and cleaned up by an SAX solid phase extraction (SPE) column. The analysis was performed on C18 column using 0.1 mol/L phosphate as the mobile phase. The recoveries of spiked samples at three levels ranging from 0.3 to 50 mg/100 g were higher than 90%. The limit of quantification was 0.3 mg/100 g. This method was accurate, simple, reproducible, and suitable for the analysis of nucleotides in milk powder.

Key words: high performance liquid chromatography (HPLC); nucleotides; SAX SPE column; milk powder

摘要： 建立乳粉中核苷酸高效液相色谱的测定方法.采用超纯水作为提取剂,0.5％的乙酸沉淀蛋白,SAX固相萃取小柱净化,C18色谱柱分离,流动相为0.1 mol/L的磷酸二氢钾溶液.每100 g乳粉中核苷酸添加量为0.3～50 mg时,回收率大于90％,定量限为0.3 mg/100 g.该方法具有结果准确、操作简便、重复性好等优点,可用于乳粉中核苷酸的检测.

关键词: 高效液相色谱法；核苷酸；SAX固相萃取小柱；乳粉