Abstract: This research evaluated the stability of vitamins and active components under ultra-low temperature conditions in order to provide a basis to formulate the quality standard for breast milk bank to ensure minimal nutrient loss during storage at ?80 ℃. The results showed that vitamin contents significantly reduced with storage time up to 12 months, yet no significant change was observed in nucleotide contents. After 3 months of storage, the contents of VB2 and VA decreased to 35% and 29% of the original level, respectively, while VC was at undetectable level. Moreover, nicotinic acid at the end of storage dropped to 69% of the fresh breast milk. Considering the existing quality indicators, the quality standard for breast milk bank should be based on breast milk components to be analyzed and fresh breast milk should be used as far as possible to analyze ingredients with poor stability such as vitamins.

Key words: human milk; vitamins; active components; stability

摘要： 通过对母乳维生素及活性成分在超低温冷冻条件下的稳定性研究,最终制定伊利母乳研究实物库的母乳质量标准,以确保伊利母乳研究实物库中的母乳样本在-80℃保存过程中营养成分的损失率最小.结果表明:母乳在-80℃冷冻保存12个月的过程中,随着保存时间的延长,维生素含量显著降低,活性成分核苷酸含量没有发生显著性变化.母乳-80℃保存3个月时,VB2和VA的含量分别减少为新鲜母乳的35％和29％,VC含量无法检出;母乳在-80℃保存12个月时,烟酸含量减少为新鲜母乳的69％.因此,从现有检测指标来看,母乳库的质量标准应根据分析研究的母乳成分而定,若分析稳定性较差的营养成分如维生素应尽量使用新鲜母乳.

关键词: 母乳；维生素；活性成分；稳定性