Progress in Research on Extracellular Proteases Produced by Psychrotrophic Pseudomonas in Milk

doi:10.15922/j.cnki.jdst.2016.01.007

Journal of Dairy Science and Technology ›› 2016, Vol. 39 ›› Issue (1): 24-27.DOI: 10.15922/j.cnki.jdst.2016.01.007

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Progress in Research on Extracellular Proteases Produced by Psychrotrophic Pseudomonas in Milk

XU Yu, YOU Chunping

(State Key Laboratory of Dairy Biotechnology, Bright Dairy Research Institute, Bright Dairy & Food Co. Ltd., Shanghai 200436, China)

Online:2016-01-01 Published:2022-02-07

牛乳中嗜冷假单胞菌胞外蛋白酶的研究进展

徐煜, 游春苹

乳业生物技术国家重点实验室,光明乳业股份有限公司光明乳业研究院,上海200436

基金资助:
“十二五”国家科技支撑计划项目(2012BAD12B08); 上海市科委优秀技术带头人计划项目(15XD1520300)

Abstract

Abstract: Pseudomonas is highlighted as predominant psychrotrophic spoilage bacteria in milk and dairy products. Its extracellular proteases are heat-resistant, and have residual activity even after ultra high temperature sterilization (UHT) treatment leading to short life of UHT milk. The current article summarizes recent studies on extracellular proteases of psychrotrophic Pseudomonas in milk, with special emphasis on their catalytic mechanisms as well as the application of protease genes including apr X to identify Pseudomonas. At last, the further research directions in this area are discussed.

Key words: psychrotrophic bacteria; Pseudomonas; protease; apr X gene

摘要： 假单胞菌通常被认为是危害原料乳及其制品的主要嗜冷菌,其分泌的胞外蛋白酶具有耐热性,甚至能在超高温灭菌处理后仍有活性残留,并减短超高温灭菌乳的货架期.本文对来自牛乳中的嗜冷假单胞菌蛋白酶的研究进展做了总结,重点假单胞菌蛋白酶的催化机制研究进展,相关基因包括蛋白酶基因apr X在假单胞菌鉴别中的应用研究进展.最后讨论了嗜冷假单胞菌蛋白酶需要深入研究的方向.

关键词: 嗜冷菌；假单胞菌；蛋白酶；基因apr X

CLC Number:

TS252.1

XU Yu, YOU Chunping. Progress in Research on Extracellular Proteases Produced by Psychrotrophic Pseudomonas in Milk[J]. Journal of Dairy Science and Technology, 2016, 39(1): 24-27.

徐煜, 游春苹. 牛乳中嗜冷假单胞菌胞外蛋白酶的研究进展[J]. 乳业科学与技术, 2016, 39(1): 24-27.

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Progress in Research on Extracellular Proteases Produced by Psychrotrophic Pseudomonas in Milk

牛乳中嗜冷假单胞菌胞外蛋白酶的研究进展

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