Abstract: A method for the quantitation of formaldehyde in infant formula was established by gas chromatography (GC) and validated by gas chromatography-mass spectrometry (GC-MS). The level of formaldehyde pollution in 45 commercial infant formula samples was determined by the proposed method and the risk of dietary exposure to formaldehyde through the consumption of infant formula was evaluated. The sample pretreatment conditions were optimized and the analytical method was assessed in terms of linearity, precision, accuracy, limit of detection (LOD) and limit of quantitation (LOQ). Quantitation was performed using the external standard method. The results showed that the linearity of this method was good in the range of 0–10 μg (r > 0.999), the LOD was 0.1 mg/kg, and the LOQ was 0.3 mg/kg. The average recoveries at spiked levels of 0.3, 0.6 and 3.0 mg/kg were in the range of 88.33%–96.56%, with relative standard deviation (RSD) less than 10%. The formaldehyde content increased with increasing reconstitution temperature above 40 ℃. In order to simulate the actual consumption process, the infant formula samples were detected after being reconstituted with warm water at 40 ℃. It turned out that formaldehyde was found in all samples at levels of 0.38–2.72 mg/kg. The average formaldehyde exposure of infants of different ages was 20.40 μg/kg at 0–6 months, 21.11 μg/kg at 6–12 months and 26.74 μg/kg at 12–36 months.

Key words: infant formula; formaldehyde content; gas chromatography; gas chromatography-mass spectrometry; exposure risk

摘要： 采用气相色谱定量及气相色谱-质谱确证，建立婴幼儿配方乳粉中甲醛含量的测定方法，并对网购45 份婴幼儿配方乳粉中甲醛的污染水平进行调查分析与暴露风险评估。通过优化前处理条件，考察方法的线性关系、精密度、准确度、检出限及定量限等指标，采用外标法定量。结果表明：该方法在0～10 μg范围内线性良好（r＞0.999），方法检出限为0.1 mg/kg，定量限为0.3 mg/kg；0.3、0.6、3.0 mg/kg加标水平下的平均加标回收率为88.33%～96.56%，相对标准偏差小于10%；当冲调温度高于40 ℃，

关键词: 婴幼儿配方乳粉；甲醛含量；气相色谱；气相色谱-质谱；暴露风险