Abstract: An ultra performance liquid chromatography-tandem mass spectrometry method for the simultaneous quantitative determination of four cephalosporin residues in dairy products, including milk, yogurt and milk powder, has been established. The samples were extracted with 5% formic acid acetonitrile. After being purified by solid-phase microextraction using an Oasis PRIME HLB cartridge, the extract was blown to dryness under a stream of nitrogen gas and then re-dissolved. The analysis was carried out using a positive electrospray ion source in the multiple reaction monitoring mode. The limit of quantification was 32 μg/kg for cefalexin, cefapirin, cefalonium and cefquinome?in milk powder; the limit of quantification was 4 μg/kg in milk and yogurt. The recoveries at spiked concentration levels of 4-150 μg/kg were 63.3%-112.1%, with relative standard deviations of 1.6%-10.5%. The matrix effects were 108.77%-379.91%. This method was characterized by short analysis time and was suitable for the determination of cephalosporin residues in different dairy products.

Key words: ultra performance liquid chromatography-tandem mass spectrometry; cephalosporin; veterinary residues; dairy products; Oasis PRIME HLB catridge

摘要： 建立牛乳、酸乳和乳粉3 种基质乳制品中采用超高效液相色谱-串联质谱（ultra performance liquid chromatography-tandem mass spectrometry，UPLC-MS/MS）同时检测4 种头孢菌素类药物残留的方法。以5%甲酸乙腈溶液进行提取，经Oasis PRIME HLB固相萃取柱净化，氮吹后复溶，采用电喷雾离子源正离子模式及多反应监测模式进行检测。结果表明：牛乳和酸乳中头孢氨苄、头孢匹林、头孢洛宁和头孢喹肟的定量限为4 μg/kg，乳粉中为32 μg/kg

关键词: 超高效液相色谱-串联质谱；头孢菌素类；兽药残留；乳制品；Oasis PRIME HLB固相萃取柱