Abstract: The associations between AGPAT6 gene polymorphisms and body size traits (body height, body length, chest circumference) were investigated in 549 Xinong Saanen and Guanzhong dairy goats using PCR-RFLP techniques. Four single nucleotide polymorphisms (SNPs) including 5'UTR (g.152G ＞ C), intron2 (g.8124G ＞ A), exon4 (g.9263C ＞ G) and 3’UTR (g.16436G ＞ A) were detected in four loci (P1, P2, P4 and P10) respectively. The population genetic analysis showed that P2, P4 and P10 possessed intermediate polymorphism. The association study revealed that none of the four SNPs detected had a significant effect on the body traits in the two goat breeds (P ＞ 0.05).

Key words: dairy goats; AGPAT6 gene; polymorphism; body size traits; association analysis

摘要： 利用限制性片段长度多态性聚合酶链反应技术对549只西农萨能和关中奶山羊群的AGPAT6基因遗传多态性与生长性状(体高、体长和胸围)的相关性进行研究.结果表明AGPAT6基因存在4个单核苷酸多态性:P1、P2、P4与P10位点分别位于5′UTR (g.152G＞C)、内含子2(g.8124G＞A)、外显子4(g.9263C＞G)和3′UTR(g.16436G＞A).经关联分析,P2、P4和P10三位点在西农萨能奶山羊和关中奶山羊品种中都属于中度多态,4个SNPs的不同基因型对两个奶山羊品种的生长性状影响均不显著(P＞0.05).

关键词: 奶山羊；AGPAT6基因；多态性；生长性状；关联分析