Abstract: A high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) was established to analyze glucose, sucrose, lactose, galactooligotrisaccharide, 1-kestose, galactooligopentosaccharide, nystose and 1F-fructofuranosyl nystose in four commercial brands of infant formula. The spiked recoveries of HPAEC-PAD ranged from 78.0% to 96.8% with relative standard deviation (RSD) of 4% (n = 5). Its limits of detection were between 3.0 and 12.0 mg/100 g. When HPAEC-PAD and the high-performance liquid chromatography (HPLC) specified in the Chinese national standard were applied to detect sucrose, lactose and 1-kestose in real samples, consistent results were obtained. However, the five other sugars were detected by HPAEC-PAD but not by HPLC. Accordingly, compared with HPLC, HPAEC-PAD was more sensitive and useful for the simultaneous determination of multiple sugars in infant formula.

Key words: ion chromatography, pulsed amperometric detection, high-performance liquid chromatography, infant formula, sugar

摘要： 采用高效阴离子交换分离-脉冲安培检测法建立婴幼儿配方乳粉中葡萄糖、蔗糖、乳糖、低聚半乳糖三糖、蔗果三糖、低聚半乳糖五糖、蔗果四糖及蔗果五糖的分析方法，对4 种品牌乳粉中的糖类进行定性和定量分析。结果表明：离子色谱法测定结果的相对标准偏差小于4%（n=5），加标回收率为78.0%～96.8%，检出限为3.0～12.0 mg/100 g；采用国标中高效液相色谱法与离子色谱法进行比较，2 种方法对乳粉中蔗糖、乳糖、蔗果三糖的检出结果基本相同，但其他5 种糖高效液相色谱法均未检出，而离子色谱法均检出相应含量，离子色谱法比高效液相色谱法灵敏度高，可同时检测婴幼儿配方乳粉中多种糖类。

关键词: 离子色谱, 脉冲安培检测, 高效液相色谱, 婴幼儿配方乳粉, 糖类