Abstract: The uncertainty in the determination of aerobic plate count in raw milk was evaluated and the feasibility of the uncertainty for judging the conformity of the test results. According to the two Chinese standards National Food Safety Standard Food Microbiological Examination: Aerobic Plate Count (GB 4789.2-2016) and Evaluation and Expression of Uncertainty in Measurement (JJF 1059.1-2012), uncertainty evaluation was carried out on 10 batches of raw milk. The extended uncertainty for the 10 batches was 0.031 0-0.033 6 (lg(CFU/mL)). The established method could be used for uncertainty assessment of aerobic plate count detection in raw milk, and the obtained uncertainty could be used for assessing the quality of raw milk.

Key words: raw milk; aerobic plate count; uncertainty

摘要： 为研究原料乳中菌落总数不确定度评定方法，对测量不确定度用于测试结果符合性判定的可行性进行讨 论。依据GB 4789.2—2016《食品安全国家标准 食品微生物学检验 菌落总数测定》和JJF 1059.1—2012《测量不确 定度评定与表示》对10 批原料乳中菌落总数的测量不确定度进行评定。结果表明，10 批原料乳中菌落总数的扩展 不确定度为0.031 0～0.033 6 （lg（CFU/mL））。本研究建立的方法可以对原料乳的菌落总数测定过程进行不确定 度评定，评定所得的不确定度适用于对原料乳进行符合性判定。

关键词: 原料乳；菌落总数；不确定度