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Journal of Dairy Science and Technology ›› 2016, Vol. 39 ›› Issue (3): 1-3.DOI: 10.15922/j.cnki.jdst.2016.03.001

• Basic Research •     Next Articles

Acid and Bile Salt Tolerance of Exopolysaccharide-Producing Strains ofLactobacillus plantarum

ZHAO Xiaoxi, WEI Xudan, CHEN Dailing, GUAN Wen, FENG Meiqin   

  1. (College of Animal Science and Technology, Jinling Institute of Technology, Nanjing 210038, China)
  • Online:2016-05-01 Published:2022-02-07

产多糖植物乳杆菌的耐酸耐胆盐能力

赵小茜, 魏旭丹, 陈戴玲, 管文, 冯美琴   

  1. 金陵科技学院动物科学与技术学院,江苏南京,210038
  • 基金资助:
    江苏省高等学校大学生创新创业训练计划项目(201313573042X;201413573045X;201513573013Z);金陵科技学院博士基金项目(jit-b-201301)

Abstract: The probiotic effects of lactic acid bacteria (LAB) depend on their tolerance to the human gastro-intestinal tract. The acid and bile salt tolerance of four exopolysaccharide-producing strains of Lactobacillus plantarum was examined by the measurement of optical density at 600 nm (OD600 nm) and the plate colony counting. The results showed that the strain F401④ had higher acid tolerance capacity, and F201⑥ had the highest bile salt tolerance capacity which showed more than 100% survival rate after culture for 4 h in the presence of 0.3 and 0.5 g/100 mL bile salt.

Key words: Lactobacillus plantarum; acid tolerance; bile salt tolerance

摘要: 乳酸菌对胃肠道的耐受性是其发挥益生功效的前提。实验采用测OD600 nm值、平板菌落计数法等方法对4株产胞外多糖的植物乳杆菌的耐酸耐胆盐能力进行了测定。结果表明:菌株F401④的耐酸性能较好;菌株F201⑥的耐胆盐性能较好,在胆盐质量浓度为0.3、0.5 g/100 mL条件下培养4 h后的存活率都超过了100%。

关键词: 植物乳杆菌;耐酸性;耐胆盐性

CLC Number: 

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