Abstract: A method for the determination of lactulose in milk powder by high performance liquid chromatography (HPLC) was developed. Lactulose was extracted with water. The analysis was performed on a NH2 column using 80% acetonitrile in water as the mobile phase. The recovery of lactulose spiked into milk powder in the range of 0.3–2.0 g/100 g was 97.4%– 103.0%. The limit of detection was 0.3 g/100 g. The results indicated that this method is accurate, simple and suitable for the analysis of lactulose in milk powder.

Key words: high performance liquid chromatography (HPLC); lactulose; milk powder

摘要： 建立乳粉中异构化乳糖高效液相色谱的测定方法.采用盐酸沉淀蛋白,氨基色谱柱分离,流动相为80％乙腈溶液,示差检测器检测.每100 g乳粉中异构化乳糖添加量为0.3～2.0 g时,回收率97.4％～103.0％.定量检出限为0.3 g/100 g.该方法操作简便,检测准确,可用于乳粉中异构化乳糖的检测.

关键词: 高效液相色谱法；异构化乳糖；乳粉