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乳业科学与技术 ›› 2020, Vol. 43 ›› Issue (5): 22-25.DOI: 10.15922/j.cnki.jdst.2020.05.005

• 分析检测 • 上一篇    下一篇

高效液相色谱法测定乳粉中玉米黄质含量

胡雪, 段国霞, 宫慧丽, 刘春霞, 陈静, 武伦玮, 刘丽君, 李翠枝, 吕志勇   

  1. 内蒙古伊利实业集团股份有限公司, 内蒙古 呼和浩特 010110
  • 出版日期:2020-09-01 发布日期:2021-11-02

Determination of Zeaxanthin in Milk Powder by High Performance Liquid Chromatography

HU Xue, DUAN Guoxia, GONG Huili, LIU Chunxia, CHEN Jing, WU Lunwei, LIU Lijun, LI Cuizhi, Lü Zhiyong   

  1. (Inner Mongolia Yili Industrial Group Co. Ltd., Hohhot 010110, China)
  • Online:2020-09-01 Published:2021-11-02

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摘要： 建立乳粉中玉米黄质含量的高效液相色谱测定方法。室温条件下，乳粉样品经氢氧化钾溶液皂化，使玉米黄质游离，再以乙醚-正己烷-环己烷混合溶剂提取，冷冻脱脂，采用梯度洗脱程序进行洗脱，C30色谱柱分离，紫外检测器检测，外标法定量。结果表明：乳粉中玉米黄质的检出限为30 μg/100 g，定量限为100 μg/100 g；当乳粉中玉米黄质加标量为90～811 μg/100 g时，加标回收率为85.8%～102.2%，相对标准偏差为1.43%～2.76%。该方法具有较好的准确度、精密度和重复性，灵敏度较高。

关键词: 玉米黄质, 高效液相色谱, 皂化, 乳粉

Abstract: A method for the determination of zeaxanthin in milk powder was developed using high performance liquid chromatography (HPLC). The sample was saponified with potassium hydroxide solution to release zeaxanthin, extracted with a mixed solvent of ethyl ether, n-hexane and cyclohexane and frozen for degreasing. The chromatographic separation was performed on a C30 column with gradient elution. The analyte was detected with an ultraviolet detector and quantified by an external standard method. The limit of quantification was 100 μg/100 g and the limit of detection was 30 μg/100 g for zeaxanthin in milk powder. The method recoveries at spiked concentration levels of 90–811 μg/100 g were 85.8%–102.2%, with relative standard deviations ranging from 1.43% to 2.76%. This method was accurate, repeatable, precise and sensitive.

Key words: zeaxanthin, high performance liquid chromatography, saponification, milk powder

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