乳业科学与技术 ›› 2016, Vol. 39 ›› Issue (4): 7-12.DOI: 10.15922/j.cnki.jdst.2016.04.002

• 基础研究 • 上一篇    下一篇

人初乳与牛初乳中乳清蛋白组成的对比研究

叶清, 石佳鑫, 杨梅, 孔颜, 叶馨阳, 梁肖娜, 乌兰君, 曹雪妍, 武俊瑞   

  1. 沈阳农业大学食品学院,辽宁沈阳,110866
  • 出版日期:2016-07-01 发布日期:2022-02-07
  • 基金资助:
    “十二五”农村领域国家科技计划项目(2013BAD18B03-02)

Comparative Analysis of Whey Protein Composition of Human and Bovine Colostrum

YE Qing, SHI Jiaxin, YANG Mei, KONG Yan, YE Xinyang, LIANG Xiaona, WU Lanjun, CAO Xueyan WU Junrui, WU Rina, LIU Biao, YUE Xiqing   

  1. (1. College of Food, Shenyang Agricultural University, Shenyang 110866, China; 2. Inner Mongolia Yili Industrial Co. Ltd., Hohhot 010050, China)
  • Published:2016-07-01 Online:2022-02-07

摘要: 通过十二烷基硫酸钠-聚丙烯酰氨凝胶电泳(sodium dodecyl sulfate polyacrylamide gel electrophoresis,SDS-PAGE)将人初乳与牛初乳中乳清蛋白进行分离并结合液质联用技术鉴定,在人初乳乳清蛋白中鉴定出477种蛋白质,牛初乳乳清蛋白中鉴定出325种蛋白质,其中人初乳中有343种特异性表达蛋白质,牛初乳中有191种特异性表达蛋白质,两者有134种相同表达蛋白质.通过基因本体(gene ontolpgy,GO)功能注释分析发现,人初乳乳清蛋白在生物过程中发挥的作用高于牛初乳,尤其表现在应激反应功能上;在分子功能上,人初乳乳清蛋白主要体现在结合作用方面;在细胞组成上,与牛初乳乳清蛋白相比,人初乳乳清蛋白参与的细胞组成均较多,其中在细胞胞外区的组成上参与最多.通过京都基因与基因组百科全书系统(Kyoto Encyclopedia of Genes and Genomes,KEGG)代谢通路分析可知,人初乳乳清蛋白中有23种蛋白参与了与消化吸收相关的KEGG通路-酶酵解.

关键词: 人乳;牛乳;乳清蛋白;功能分析;KEGG代谢通路

Abstract: In this study, the whey proteins in human and bovine colostrum were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and identified by liquid chromatography-mass spectrometry (LC-MS). A total of 477 whey proteins were identified in human colostrum and 325 in bovine colostrum. Totally, 343 proteins were specifically expressed in human colostrum and 191 in bovine colostum. Moreover, 134 specifically expressed proteins were common to both. Gene ontology (GO) annotation analysis revealed that human colostrum whey proteins played a more important role in bioprocesses especially stress responses than their bovine counterparts. Human colostrum whey proteins exerted functions by binding to other molecules. Human colostrum whey proteins were more important constituents of cellular structures as compared with their bovine counterparts, and they were the most significant constituents of extracellular structures. According to the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database, 23 whey proteins in human colostrum were involved in the KEGG pathway related to digestion and absorption, enzymatic glycolysis.

Key words: human colostrum; bovine colostrum; milk whey protein; functional analysis; KEGG pathway

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