Abstract: Biotin is one of the essential vitamins for maintaining normal physiological functions of the animal organism. This paper provides a review on microbiological, chromatographic and mass spectrometric assays for free biotin in dairy products. Their advantages and disadvantages as well as points for attent2ion during the operation are compared. Guidelines on how to select the appropriate assay in the industrial production and future trends in the analysis of biotin are put forward.

Key words: biotin；assay；milk products

摘要： 生物素是动物机体维持正常生理机能所必需的维生素之一。本文综述了乳制品中游离生物素分析的微生物法、色谱法及质谱法,比较了各种方法的优缺点及操作中的注意事项,阐述了企业如何选择生物素分析方法及未来生物素分析的发展趋势。

关键词: 生物素;检测方法;乳制品