Abstract: ：An enrichment culture medium has been developed for the industrial mass production of lactic acid bacteria. Onefactor- at-a-time experiments were conducted to choose optimal carbon and nitrogen sources and growth factor, and their levels were optimized using an orthogonal array design. Our results demonstrated that the optimal medium composition consisted of 10% pineapple peel juice, 25% Pleurotus ostreatus soup and 20% potato juice. The enrichment culture medium was not only significantly more effective in enriching lactic acid bacteria than MRS medium but also inexpensive, thus favoring the industrial mass production of lactic acid bacteria.

Key words: Lactobacillus bulgaricus；Streptococcus thermophilus；carbon source；nitrogen source；growth factors

摘要： 通过单因素试验选出乳酸菌增菌培养基的最佳的碳源、氮源和生长因子,然后利用正交试验选出最佳的乳酸菌的增菌培养基。结果表明：10%菠萝皮汁、25%平菇浸汁和20%马铃薯汁配制而成的乳酸菌培养基的增菌效果最佳,这种培养基不仅增菌效果明显优越于MRS培养基,而且价格低廉,为乳酸菌的工业化大规模生产提供了有利的条件。

关键词: 保加利亚乳杆菌;嗜热链球菌;碳源;氮源;生长因子