Abstract: Objective: To develop a colloidal gold-based immunochromatographic assay for rapid detection of casein. Methods: We used casein and anti-casein polyclonal antibody as raw materials, nitrocellulose membrane and glass fiber paper as a carrier to prepare a rapid test strip. Results: The sensitivity of the test strip was 2.3 mg/mL, and the detection process required 10 min with good repeatability and stability. Conclusion: We have successfully developed a casein test strip.

Key words: casein；polyclonal antibody；colloidal gold-based immunochromatographic assay；rapid detection

摘要： 目的:制备检测乳制品中酪蛋白含量的胶体金快速检测卡.方法:以酪蛋白为抗原和抗酪蛋白多克隆抗体为原料,用硝酸纤维膜和玻璃纤维纸作为载体,制作出检测酪蛋白胶体金快速检测卡.结果:该检测卡检测酪蛋白的灵敏度可达到2.3mg/mL,检测时间10min,重复性和稳定性良好.结论:本实验成功制作出了能检测酪蛋白的快速检测卡.

关键词: 酪蛋白；多克隆抗体；胶体金免疫层析；快速检测