Abstract: A method for determining lutein in dairy products by HPLC is described. Samples were extracted with acetone and separated with a mobile phase made up of methanol and methyltert-butyl ether. The analysis was performed by UV detection at 445 nm, and the external standard method was used for quantification. This method has good accuracy and precision. The spiked recoveries in milk and milk powder ranged from 86.9% to 101.2% with a relative standard deviation of 2.56% to 4.05%. The detection limit was 0.02 mg/kg, and a linear range of 0.02 to 4 mg/kg was obtained.

Key words: HPLC; lutein; dairy products

摘要： 建立了乳制品中的叶黄素液相色谱分析方法.样品经丙酮提取后,以甲醇-甲基叔丁基醚为流动相,反相色谱分离,紫外检测器445 nm进行检测,外标法定量.该方法具有良好的准确度和精密度,回收率为86.9％～101.2％,相对标准偏差为2.56％～4.05％,定量检出限0.02 mg/kg,线性范围0.02～4 mg/kg.

关键词: 商效液相色谱法；叶黄素；乳制品