Abstract: Lactoferrin is an important multifunctional protein that has a variety of biological activities, such as anti-bacterial, anti-tumor, anti-allergy, immunoregulatory and so on. The related basic research and application development of lactoferrin have attracted wide attention. Therefore, the detection methods for lactoferrin have drawn increasing attention. Especially the advantages, sensitivity, accuracy and stability of different methods vary to different extents, which directly affects their applicable scope. The present paper has described the detection methods which have been reported to be suitable for different types of biological samples, mainly including spectrophotometry, high performance liquid chromatography (HPLC), enzyme-linked immunosorbent assay (ELISA), high affinity chromatography (HPAC), efficient capillary electrophoresis (HPCE) and surface plasmon resonance (SPR). The advantages and disadvantages of these different detection technologies also have been analyzed and compared. This review is expected to provide reference for selection of the suitable detection method for lactoferrin in research and development practices.

Key words: lactoferrin; detection methods; progress

摘要： 乳铁蛋白是一种重要的多功能蛋白,具有抗菌、抗肿瘤、抗过敏、调节免疫等多种生物学活性,相关基础研究及产品开发已经引起了广泛关注.因此,乳铁蛋白检测方法也备受关注.尤其是不同方法各自的优势、灵敏度、准确度及稳定性等方面具有一定的差异,这也直接影响着该方法的适用范围.本研究对适合不同类型生物样品中乳铁蛋白的检测技术进行了阐述,主要有分光光度法、高效液相色谱法、酶联免疫吸附法、高效亲和色谱法、高效毛细血管电泳法以及表面等离子共振技术等检测技术,并分析比较了这些检测技术的不同优缺点,以期能为乳铁蛋白的研究与开发实践中选择恰当的检测方法提供一定的参考.

关键词: 乳铁蛋白；检测方法；研究进展