Abstract: A method was developed for the simultaneous determination of 2 ultraviolet absorbers UV-326, UV-1577 and 2 antioxidants AO-1010 and AO-2246 in milk by high performance liquid chromatography. Samples were extracted with acetonitrile. The effective separation of the target analytes was accomplished on Agilent HC-C8 (150 mm × 4.6 mm, 5.0 μm) column with gradient elution procedure using a mixture of water and acetonitrile as the mobile phase. External calibration was used for quantitative determination at detection wavelength of 280 nm. The linearity of 4 analytes were satisfactory with a correlation coefficient of > 0.9995 at concentrations ranging from 0.05 to 50 μg/mL. The limits of detection of the method were 0.02?0.5 mg/kg, and the recoveries at different spiked levels and precision (RSD) were 69.08%?115.87% and 0.43%?9.94%, respectively. The method is simple, sensitive, reproducible and suitable for the simultaneous determination of 2 ultraviolet absorbers and 2 antioxidants in milk.

Key words: high performance liquid chromatography (HPLC); ultraviolet absorber; antioxidants; milk

摘要： 建立液态乳中2种紫外吸收剂(UV-326和UV-1577)和2种抗氧化剂(AO-1010、AO-2246)的高效液相色谱测定方法.前处理采用乙腈提取液态乳中目标化合物,样品用正己烷提取;检测经Agilent HC-C8反相色谱柱,以乙腈-水作为流动相,梯度洗脱程序进行分离,280 nm波长下检测.结果表明:分析物在0.05～50 μg/mL质量浓度范围内线性关系良好,线性相关系数＞0.999 5,方法检出限为0.02～0.5 mg/kg.方法在3个水平的加标回收率为69.08％～115.87％,相对标准偏差为0.43％～9.94％.该方法操作简单、灵敏度高、重现性好,适用于液态乳中紫外吸收剂和抗氧化剂的同时测定.

关键词: 高效液相色谱；紫外吸收剂；抗氧化剂；液态乳