Determination of Hippuric Acid and Benzoic Acid in Dairy Products by High Performance Liquid Chromatography

doi:10.15922/j.cnki.jdst.2017.02.006

Abstract

Abstract: A method for determination of hippuric acid and benzoic acid in dairy products by high performance liquid chromatography (HPLC) is described.The protein samples was precipitated by adding lead acetate and centrifuged,and then the analytes were separated on a C18 column with a mobile phase consisting of ammonium acetate and methanol solution and detected with a UV detector at 230 nm.The results showed that hippuric acid and benzoic acid displayed a good linearity relationship in the concentration range of 1-100 mg/kg.The recovery rate was 95.3％-103.6％.The method proved to be simple,accurate and repeatable.

Key words: high performance liquid chromatography (HPLC); hippuric acid; benzoic acid; dairy products

摘要： 建立乳制品中马尿酸和苯甲酸的高效液相色谱(high performance liquid chromatography,HPLC)分析方法。样品用乙酸铅沉淀过滤后,C18反相色谱柱分离,以乙酸铵-甲醇溶液为流动相,采用紫外检测器于230 nm波长处检测。结果表明:马尿酸和苯甲酸在1～100 mg/kg范围内呈良好的线性关系,回收率为95.3％～103.6％。本方法用于乳制品中马尿酸和苯甲酸的测定,前处理简单、定量准确,且重复性好。

关键词: 高效液相色谱法; 马尿酸; 苯甲酸; 乳制品

CLC Number:

TS252.7

ZHAO Zhen, LIU Chunxia, LIU Lijun, YUE Hong, WAN Peng, LI Cuizhi. Determination of Hippuric Acid and Benzoic Acid in Dairy Products by High Performance Liquid Chromatography[J]. Journal of Dairy Science and Technology, 2017, 40(2): 27-29.

赵贞, 刘春霞, 刘丽君, 岳虹, 万鹏, 李翠枝. 高效液相色谱法检测乳品中的马尿酸和苯甲酸[J]. 乳业科学与技术, 2017, 40(2): 27-29.

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Determination of Hippuric Acid and Benzoic Acid in Dairy Products by High Performance Liquid Chromatography

高效液相色谱法检测乳品中的马尿酸和苯甲酸

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