Abstract: This study was an attempt to optimize the formulation of a shelf-stable fermented milk beverage using response surface methodology. The effect of whole milk powder-to-whey ratio, agar, starch, pectin, fructose and cream on the viscosity and water-holding capacity of the beverage was investigated. Whole milk powder-to-whey ratio, agar and starch were identified as the most significant factors using the Plackett-Burman design (P < 0.05). The levels of the four factors were optimized using response surface methodology with Box-Behnken design. A response surface model was developed indicating the relationship between each of the response variables and the four factors. The optimal combination obtained from the model was whole milk powder-to-whey ratio 5:1, pectin 1 g/L, starch 10 g/L and pectin 0.5 g/L.

Key words: shelf-stable fermented milk beverage yogurt; response surface methodology; Plackett-Burman design; viscosity; water-holding capacity

摘要： 采用响应曲面法对常温饮用型酸乳的配方进行优化。用Plackett-Burman设计法研究全脂乳粉与乳清粉的蛋白比例、琼脂、淀粉、果胶、果糖和稀奶油添加量6 个因素对酸乳黏度及持水力的影响，筛选出影响酸乳黏度和持水力的显著因素，用Box-Behnken试验设计优化最佳配方。结果表明：全脂乳粉与乳清粉的蛋白比例、琼脂、淀粉及果胶添加量是影响常温饮用型酸乳黏度和持水力的显著因素（P＜0.05）；经过响应面试验设计建立黏度、持水力和4因素的模型，对此模型进行求解得到最佳配方为全脂乳粉与乳清粉的蛋白比例5∶1、琼脂

关键词: 常温饮用型酸乳；响应面分析；Plackett-Burman设计；黏度；持水力