Abstract: Constructing an efficient and safe producer strain is a prerequisite for biosynthetic production of 2’-fucosyllactose. The number and technical content of recent patents on 2’-fucosyllactose biosynthesis applied for in China are reviewed in this paper, indicating that over 80 percent of the patents focused on the construction of recombinant strains by optimizing the metabolic pathways, overexpressing 2’-fucosyllactose biosynthesis-related enzyme genes, and developing more safe and efficient host strains. However, after a review of China’s patented technologies in this regard, we found that research on the biosynthetic production of 2’-fucosyllactose in a pilot scale or above is weak, and there is a lacuna in utility and industrially oriented patented technologies, which will bottleneck the 2’-fucosyllactose biosynthesis industry in China.

Key words: 2’-fucosyllactose, recombinant strain, gene editing, fermentation, patent

摘要： 生物发酵法生产2’-岩藻糖基乳糖（2’-fucosyllactose，2’-FL），首要条件是构建出安全、高效的生产菌株。本文分析近年申请我国生物合成2’-FL相关专利的数量和技术内容，发现80%以上的专利内容围绕重组菌的构建，通过优化代谢途径、过表达2’-FL合成中相关酶的基因，寻找更安全、高效的宿主菌。然而，在我国的专利技术中，基于中试规模及以上的研究比较薄弱，实用型和面向产业化的专利技术几乎空白，这将是制约我国工业化生物合成2’-FL的瓶颈。

关键词: 2’-岩藻糖基乳糖, 重组菌, 基因编辑, 发酵, 专利