[an error occurred while processing this directive]

乳业科学与技术 ›› 2020, Vol. 43 ›› Issue (1): 5-8.DOI: 10.15922/j.cnki.jdst.2020.01.002

• 分析检测 • 上一篇    下一篇

高效液相色谱-串联质谱法测定特殊医学用途婴儿乳粉中牛磺酸的含量

林琳, 林毅侃, 庞贝妮, 翁史昱   

  1. 上海市质量监督检验技术研究院, 国家食品质量监督检验中心(上海), 上海 200233
  • 出版日期:2020-01-01 发布日期:2021-11-02
  • 基金资助:
    上海市食品质量安全检测与评价专业技术服务平台项目（18DZ2292400）

Determination of Taurine Content in Infant Formula for Special Medical Purposes by High Performance Liquid Chromatography-Tandem Mass Spectrometry

LIN Lin, LIN Yikan, PANG Beini, WENG Shiyu   

  1. (National Food Quality Supervision and Inspection Center (Shanghai), Shanghai Institute of Quality Inspection and Technical Research, Shanghai 200233, China)
  • Online:2020-01-01 Published:2021-11-02

RichHTML

PDF (PC)

摘要： 建立高效液相色谱-串联质谱法测定特殊医学用途婴儿乳粉中牛磺酸含量的分析方法。乳粉样品加水振荡15 min提取牛磺酸，5%偏磷酸沉淀样品中的蛋白质；采用SAX色谱柱（2.1 mm×150 mm，3.5 μm）分离，以乙腈-0.1%甲酸水溶液体系为流动相进行洗脱，在电喷雾离子源正离子模式下电离，多反应模式监测。结果表明：牛磺酸在6.44 min出峰良好，在0.2～2.0 mg/L范围内线性良好（R2＞0.999），检出限为0.3 mg/100 g，定量限为1.0 mg/100 g；牛磺酸在低、中、高3 个加标水平下的平均回收率为90.1%～102.4%，相对标准偏差为0.39%～3.30%。该方法具有灵敏度及准确度高、重复性和稳定性好等优点，适用于特殊医学用途婴儿乳粉中牛磺酸的有效检测。

关键词: 高效液相色谱-串联质谱法, 特殊医学用途婴儿乳粉, 牛磺酸

Abstract: An analytical method for the determination of taurine content in infant formula for special medical purposes has been established using liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Samples were dissolved in double distilled water with constant shaking for 15 min for the purpose of taurine extraction, followed by protein precipitation with 5% metaphosphoric acid. The analyte was separated on an SAX column (2.1 mm × 150 mm, 3.5 μm) using gradient elution with a mobile phase containing 0.1% (V/V) formic acid in water and acetonitrile. The chromatographic separation was completed within 9 min. Thereafter, the analysis was performed using an electrospray ionization source in the positive ion mode with multireaction monitoring. As a result, good linearity was observed in the concentration range from 0.2 to 2.0 mg/L with correlation coefficient (R2) of greater than 0.999. The limit of detection (LOD) was 0.3 mg/100 g, and the limit of quantification (LOQ) was 1.0 mg/100 g. The average recoveries at three different spiked concentration levels ranged from 90.1% to 102.4% with relative standard deviation of 0.39%–3.30% (n = 6). This method is applicable to analyze the taurine content in infant formula for special medical purposes, owing to its good sensitivity, recovery, repeatability and stability.

Key words: high performance liquid chromatography-tandem mass spectrometry, infant formula for special medical purposes, taurine

中图分类号: 

[an error occurred while processing this directive]